Bioengineering efficient antibiotic biosynthesis in E.
The paths based on the past production of antibiotics are now quite well known. For example, the antibacterial activity of erythromycin, an important antibiotic polyketide precursor, which requires transfer of two unusual sugars called mycarose and desosamine (both glycosyl groups) in the non-sweet glycosides of the molecule (macrocyclic aglycone). In a new study published online this week in PLoS Biology Open Access uses Ho Young Lee and Chaitan Khosla bioassay to demonstrate how the development of this route of antibiotics in Escherichia coli (E.coli to be determined) is more efficient antibiotic-producing mutants.The authors reconstituted the biosynthetic pathways of sugars in E. coli to produce the antibiotic 6 deoxyerythromycin D. During the preparation of a
recombinant strain of E. coli that produces the bioactive macrolide 6-D deoxyerythromycin propionate, which has developed a completely new instrument to improve the efficiency of biosynthetic engineering of this class of antibiotics. Initially, this recombinant strain produced barely enough antibiotic activity to establish a screening test based on the work.Therefore, the authors use the test for the detection of over-production of antibiotics. After three rounds of selection, were able to identify the cells of E. coli that the antibiotic produced in excess of 6 D deoxyerythromycin mycarose with significant changes in the biosynthetic pathway. They used the activity based on the detection system itself to evolve mutants of E. coli capable of precursor in the biosynthesis of more
efficient run. As the first example of biological evolution has led to a course of antibiotics in E. coli, these results open the door to harness the power of genetic research in the field of polyketide synthases and also for the mechanism of biosynthesis.---------------------------- Article adapted by Medical News Today from original press release. ---------------------------- Note: Lee HY, Khosla C (2007) The biological
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